Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB.

نویسندگان

  • Suk-Chae Jung
  • Chris L Smith
  • Ki-Sung Lee
  • Min-Eui Hong
  • Dae-Hyuk Kweon
  • Gregory Stephanopoulos
  • Yong-Su Jin
چکیده

A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 76 18  شماره 

صفحات  -

تاریخ انتشار 2010